Three-dimensional reconstruction of cardiac sarcoplasmic reticulum reveals a continuous network linking transverse-tubules: this organization is perturbed in heart failure. Spatial and functional relationship between myocytes and fibroblasts in the rabbit sinoatrial node. Light microscopy of proteins in their ultrastructural context. MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells. Superresolution microscopy in heart – cardiac nanoscopy. Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission. Myocardial ultrastructure and function during progressive early ischemia in the intact heart. Contributions of electron microscopy to the study of myocardial ischaemia. Ultrastructural correlates of reduced cardiac function in human heart disease. Quantitative studies on plasmalemmal folds and caveolae of rabbit ventricular myocardial cells. Its ultrastructure and the electrophysiological implications of its geometry. The correlation of ultrastructure and function in the mammalian myocardial cell. Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. Shape, size, and distribution of Ca 2+ release units and couplons in skeletal and cardiac muscles. Purification and reconstitution of the calcium release channel from skeletal muscle. Localization of Ca 2+ release channels with ryanodine in junctional terminal cisternae of sarcoplasmic reticulum of fast skeletal muscle. Structural evidence for direct interaction between the molecular components of the transverse tubule/sarcoplasmic reticulum junction in skeletal muscle. Studies of the triad: structure of the junction in frog twitch fibers. The ultrastructure of the cat myocardium. Its form and distribution in striated muscle cells. Localization of calcium-accumulating structures in striated muscle fibers. Structural basis of the cross-striations in muscle. Electron microscope study of mammalian cardiac muscle cells. Die submikroskopische Morphologie des Herzmuskels. The abrupt transition from rest to activity in muscle. Electron microscope studies on the structure of cardiac muscle. In this Review, we discuss the progress in this fascinating new field to highlight the fundamental scientific insight that has emerged, based on technological breakthroughs in biological sample preparation, 3D imaging and data analysis to illustrate the potential clinical relevance of understanding 3D cardiac nanodynamics and to predict further progress that we can reasonably expect to see over the next 10 years. In combination with functional studies, such as fluorescence imaging, exciting insights into cardiac autoregulatory processes at nano-to-micro scales are starting to emerge. By high-pressure freezing of cardiomyocytes with millisecond accuracy after initiation of an action potential, pseudodynamic snapshots of contraction-induced deformation of intracellular organelles can now be captured. These advances have provided access to a new generation of data and have driven the development of increasingly smart, artificial intelligence-based, deep-learning image-analysis algorithms. Over the past decade, the first inroads have been made towards 3D reconstruction of heart cells, with a native resolution of around 1 nm 3, and of individual molecules relevant to heart function at a near-atomic scale. The 3D nanostructure of the heart, its dynamic deformation during cycles of contraction and relaxation, and the effects of this deformation on cell function remain largely uncharted territory.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |